Skip to main content

Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis

Author(s): Szczuka, Aleksandra; Morel, François MM; Schaefer, Jeffra K

To refer to this page use:
Abstract: Mercury uptake in bacteria represents a key first step in the production and accumulation of methylmercury in biota. Previous experiments with mercury methylating bacteria have shown that Hg uptake is enhanced by some thiols, in particular cysteine, and that it is an energy-dependent process through heavy metal transporters [Schaefer et al. Environ. Sci. Technol.2014, 48, 3007]. In this study, we examine Hg uptake in the nonmethylating facultative aerobe, Shewanella oneidensis, under both anaerobic and aerobic conditions. Our results demonstrate similar characteristics of the Hg uptake system to those of the Hg methylating strains: (1) uptake is enhanced in the presence of some thiols but not others; (2) uptake is energy dependent as evidenced by inhibition by a protonophore, and (3) uptake is inhibited by high Zn(II) concentrations. Initial cellular uptake rates in S. oneidensis were remarkably similar under aerobic and fumarate-reducing conditions. These data support a similar Hg(II) uptake mechanism within the proteobacteria of accidental Hg(II) transport through heavy metal transporters with similar rates of uptake but differences in the ability to take up Hg bound to different thiols.
Publication Date: 18-May-2015
Citation: Szczuka, Aleksandra, François MM Morel, and Jeffra K. Schaefer. "Effect of thiols, zinc, and redox conditions on Hg uptake in Shewanella oneidensis." Environmental Science & Technology 49, no. 12 (2015): 7432-7438. doi:10.1021/acs.est.5b00676.
DOI: doi:10.1021/acs.est.5b00676
ISSN: 0013-936X
EISSN: 1520-5851
Pages: 7432 - 7438
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Environmental Science & Technology
Version: Final published version. This is an open access article.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.