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Noise and interlocking signaling pathways promote distinct transcription factor dynamics in response to different stresses

Author(s): Petrenko, Natalia; Chereji, Raˇzvan V; McClean, Megan N; Morozov, Alexandre V; Broach, James R

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Abstract: All cells perceive and respond to environmental stresses through elaborate stress-sensing networks. Yeast cells sense stress through diverse signaling pathways that converge on the transcription factors Msn2 and Msn4, which respond by initiating rapid, idiosyncratic cycles into and out of the nucleus. To understand the role of Msn2/4 nuclear localization dynamics, we combined time-lapse studies of Msn2-GFP localization in living cells with computational modeling of stress-sensing signaling networks. We find that several signaling pathways, including Ras/protein kinase A, AMP-activated kinase, the high-osmolarity response mitogen-activated protein kinase pathway, and protein phosphatase 1, regulate activation of Msn2 in distinct ways in response to different stresses. Moreover, we find that bursts of nuclear localization elicit a more robust transcriptional response than does sustained nuclear localization. Using stochastic modeling, we reproduce in silico the responses of Msn2 to different stresses, and demonstrate that bursts of localization arise from noise in the signaling pathways amplified by the small number of Msn2 molecules in the cell. This noise imparts diverse behaviors to genetically identical cells, allowing cell populations to “hedge their bets” in responding to an uncertain future, and to balance growth and survival in an unpredictable environment.
Publication Date: 15-Jun-2013
Citation: Petrenko, Natalia, Chereji, Raˇzvan V, McClean, Megan N, Morozov, Alexandre V, Broach, James R. (2013). Noise and interlocking signaling pathways promote distinct transcription factor dynamics in response to different stresses. Molecular Biology of the Cell, 24 (12), 2045 - 2057. doi:10.1091/mbc.e12-12-0870
DOI: doi:10.1091/mbc.e12-12-0870
ISSN: 1059-1524
EISSN: 1939-4586
Pages: 1 - 13
Type of Material: Journal Article
Journal/Proceeding Title: Molecular Biology of the Cell
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

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