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Quorum sensing controls Vibrio cholerae multicellular aggregate formation.

Author(s): Jemielita, Matthew; Wingreen, Ned S; Bassler, Bonnie L

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Abstract: Bacteria communicate and collectively regulate gene expression using a process called quorum sensing (QS). QS relies on group-wide responses to signal molecules called autoinducers. Here, we show that QS activates a new program of multicellularity in Vibrio cholerae. This program, which we term aggregation, is distinct from the canonical surface-biofilm formation program, which QS represses. Aggregation is induced by autoinducers, occurs rapidly in cell suspensions, and does not require cell division, features strikingly dissimilar from those characteristic of V. cholerae biofilm formation. Extracellular DNA limits aggregate size, but is not sufficient to drive aggregation. A mutagenesis screen identifies genes required for aggregate formation, revealing proteins involved in V. cholerae intestinal colonization, stress response, and a protein that distinguishes the current V. cholerae pandemic strain from earlier pandemic strains. We suggest that QS-controlled aggregate formation is important for V. cholerae to successfully transit between the marine niche and the human host.
Publication Date: 24-Dec-2018
Citation: Jemielita, Matthew, Wingreen, Ned S, Bassler, Bonnie L. (2018). Quorum sensing controls Vibrio cholerae multicellular aggregate formation.. eLife, 7 (10.7554/eLife.42057
DOI: doi:10.7554/eLife.42057
ISSN: 2050-084X
EISSN: 2050-084X
Pages: 1 - 25
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

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