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Post-transcriptional gene regulation by an Hfq-independent small RNA in Caulobacter crescentus.

Author(s): Fröhlich, Kathrin S; Förstner, Konrad U; Gitai, Zemer

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Abstract: Bacterial small RNAs (sRNAs) are a heterogeneous group of post-transcriptional regulators that often act at the heart of large networks. Hundreds of sRNAs have been discovered by genome-wide screens and most of these sRNAs exert their functions by base-pairing with target mRNAs. However, studies addressing the molecular roles of sRNAs have been largely confined to gamma-proteobacteria, such as Escherichia coli. Here we identify and characterize a novel sRNA, ChvR, from the alpha-proteobacterium Caulobacter crescentus. Transcription of chvR is controlled by the conserved two-component system ChvI-ChvG and it is expressed in response to DNA damage, low pH, and growth in minimal medium. Transient over-expression of ChvR in combination with genome-wide transcriptome profiling identified the mRNA of the TonB-dependent receptor ChvT as the sole target of ChvR. Genetic and biochemical analyses showed that ChvR represses ChvT at the post-transcriptional level through direct base-pairing. Fine-mapping of the ChvR-chvT interaction revealed the requirement of two distinct base-pairing sites for full target regulation. Finally, we show that ChvR-controlled repression of chvT is independent of the ubiquitous RNA-chaperone Hfq, and therefore distinct from previously reported mechanisms employed by prototypical bacterial sRNAs. These findings have implications for the mechanism and evolution of sRNA function across bacterial species.
Publication Date: Nov-2018
Citation: Fröhlich, Kathrin S, Förstner, Konrad U, Gitai, Zemer. (2018). Post-transcriptional gene regulation by an Hfq-independent small RNA in Caulobacter crescentus.. Nucleic acids research, 46 (20), 10969 - 10982. doi:10.1093/nar/gky765
DOI: doi:10.1093/nar/gky765
ISSN: 0305-1048
EISSN: 1362-4962
Pages: 1 - 14
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Nucleic acids research
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

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