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A Host-Produced Autoinducer-2 Mimic Activates Bacterial Quorum Sensing

Author(s): Ismail, Anisa S; Valastyan, Julie S; Bassler, Bonnie L

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Abstract: Host-microbial symbioses are vital to health; nonetheless, little is known about the role cross-kingdom signaling plays in these relationships. In a process called quorum sensing, bacteria communicate with one another using extracellular signal molecules called autoinducers. One autoinducer, AI-2, is proposed to promote inter-species bacterial communication, including in the mammalian gut. We show that mammalian epithelia produce an AI-2 mimic activity in response to bacteria or tight-junction disruption. This AI-2 mimic is detected by the bacterial AI-2 receptor, LuxP/LsrB, and can activate quorum-sensing-controlled gene expression, including in the enteric pathogen Salmonella typhimurium. AI-2 mimic activity is induced when epithelia are directly or indirectly exposed to bacteria, suggesting that a secreted bacterial component(s) stimulates its production. Mutagenesis revealed genes required for bacteria to both detect and stimulate production of the AI-2 mimic. These findings uncover a potential role for the mammalian AI-2 mimic in fostering cross-kingdom signaling and host-bacterial symbioses.
Publication Date: Apr-2016
Citation: Ismail, Anisa S, Valastyan, Julie S, Bassler, Bonnie L. (2016). A Host-Produced Autoinducer-2 Mimic Activates Bacterial Quorum Sensing. Cell Host & Microbe, 19 (4), 470 - 480. doi:10.1016/j.chom.2016.02.020
DOI: doi:10.1016/j.chom.2016.02.020
ISSN: 1931-3128
Pages: 1 - 25
Type of Material: Journal Article
Journal/Proceeding Title: Cell Host & Microbe
Version: Author's manuscript

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