Skip to main content

Real-time 2-5A kinetics suggest that interferons β and λ evade global arrest of translation by RNase L.

Author(s): Chitrakar, Alisha; Rath, Sneha; Donovan, Jesse; Demarest, Kaitlin; Li, Yize; et al

Download
To refer to this page use: http://arks.princeton.edu/ark:/88435/pr1c19m
Full metadata record
DC FieldValueLanguage
dc.contributor.authorChitrakar, Alisha-
dc.contributor.authorRath, Sneha-
dc.contributor.authorDonovan, Jesse-
dc.contributor.authorDemarest, Kaitlin-
dc.contributor.authorLi, Yize-
dc.contributor.authorSridhar, Raghavendra Rao-
dc.contributor.authorWeiss, Susan R-
dc.contributor.authorKotenko, Sergei V-
dc.contributor.authorWingreen, Ned S-
dc.contributor.authorKorennykh, Alexei-
dc.date.accessioned2020-02-25T20:11:41Z-
dc.date.available2020-02-25T20:11:41Z-
dc.date.issued2019-02en_US
dc.identifier.citationChitrakar, Alisha, Rath, Sneha, Donovan, Jesse, Demarest, Kaitlin, Li, Yize, Sridhar, Raghavendra Rao, Weiss, Susan R, Kotenko, Sergei V, Wingreen, Ned S, Korennykh, Alexei. (2019). Real-time 2-5A kinetics suggest that interferons β and λ evade global arrest of translation by RNase L.. Proceedings of the National Academy of Sciences of the United States of America, 116 (6), 2103 - 2111. doi:10.1073/pnas.1818363116en_US
dc.identifier.issn0027-8424-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1c19m-
dc.description.abstractCells of all mammals recognize double-stranded RNA (dsRNA) as a foreign material. In response, they release interferons (IFNs) and activate a ubiquitously expressed pseudokinase/endoribonuclease RNase L. RNase L executes regulated RNA decay and halts global translation. Here, we developed a biosensor for 2',5'-oligoadenylate (2-5A), the natural activator of RNase L. Using this biosensor, we found that 2-5A was acutely synthesized by cells in response to dsRNA sensing, which immediately triggered cellular RNA cleavage by RNase L and arrested host protein synthesis. However, translation-arrested cells still transcribed IFN-stimulated genes and secreted IFNs of types I and III (IFN-β and IFN-λ). Our data suggest that IFNs escape from the action of RNase L on translation. We propose that the 2-5A/RNase L pathway serves to rapidly and accurately suppress basal protein synthesis, preserving privileged production of defense proteins of the innate immune system.en_US
dc.format.extent1 - 37en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.rightsAuthor's manuscripten_US
dc.titleReal-time 2-5A kinetics suggest that interferons β and λ evade global arrest of translation by RNase L.en_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1073/pnas.1818363116-
dc.identifier.eissn1091-6490-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

Files in This Item:
File Description SizeFormat 
AM_Realtime_2_5A_kinetics_suggests_interferons.pdf1.08 MBAdobe PDFView/Download


Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.