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Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast

Author(s): McIsaac, R Scott; Oakes, Benjamin L; Wang, Xin; Dummit, Krysta A; Botstein, David; et al

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Abstract: A general method for the dynamic control of single gene expression in eukaryotes, with no off-target effects, is a long-sought tool for molecular and systems biologists. We engineered two artificial transcription factors (ATFs) that contain Cys2His2 zinc-finger DNA-binding domains of either the mouse transcription factor Zif268 (9 bp of specificity) or a rationally designed array of four zinc fingers (12 bp of specificity). These domains were expressed as fusions to the human estrogen receptor and VP16 activation domain. The ATFs can rapidly induce a single gene driven by a synthetic promoter in response to introduction of an otherwise inert hormone with no detectable off-target effects. In the absence of inducer, the synthetic promoter is inactive and the regulated gene product is not detected. Following addition of inducer, transcripts are induced >50-fold within 15 min. We present a quantitative characterization of these ATFs and provide constructs for making their implementation straightforward. These new tools allow for the elucidation of regulatory network elements dynamically, which we demonstrate with a major metabolic regulator, Gcn4p.
Publication Date: Feb-2013
Electronic Publication Date: 28-Dec-2012
Citation: McIsaac, R Scott, Oakes, Benjamin L, Wang, Xin, Dummit, Krysta A, Botstein, David, Noyes, Marcus B. (2013). Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast. Nucleic Acids Research, 41 (4), e57 - e57. doi:10.1093/nar/gks1313
DOI: doi:10.1093/nar/gks1313
ISSN: 0305-1048
EISSN: 1362-4962
Pages: 1 - 10
Type of Material: Journal Article
Journal/Proceeding Title: Nucleic Acids Research
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

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