Skip to main content

Enhancer Control of Transcriptional Bursting

Author(s): Fukaya, Takashi; Lim, Bomyi; Levine, Michael S.

To refer to this page use:
Abstract: Transcription is episodic, consisting of a series of discontinuous bursts. Using live-imaging methods and quantitative analysis, we examine transcriptional bursting in living Drosophila embryos. Different developmental enhancers positioned downstream of synthetic reporter genes produce transcriptional bursts with similar amplitudes and duration but generate very different bursting frequencies, with strong enhancers producing more bursts than weak enhancers. Insertion of an insulator reduces the number of bursts and the corresponding level of gene expression, suggesting that enhancer regulation of bursting frequency is a key parameter of gene control in development. We also show that linked reporter genes exhibit coordinated bursting profiles when regulated by a shared enhancer, challenging conventional models of enhancer-promoter looping.
Publication Date: Jul-2016
Citation: Fukaya, Takashi, Lim, Bomyi, Levine, Michael. (2016). Enhancer Control of Transcriptional Bursting. Cell, 166 (2), 358 - 368. doi:10.1016/j.cell.2016.05.025
DOI: doi:10.1016/j.cell.2016.05.025
ISSN: 0092-8674
EISSN: 1097-4172
Pages: 358 - 368
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Cell
Version: Author's manuscript

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.