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Nervous system-wide profiling of presynaptic mRNAs reveals regulators of associative memory

Author(s): Arey, Rachel N; Kaletsky, Rachel; Murphy, Coleen T

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Abstract: Presynaptic protein synthesis is important in the adult central nervous system; however, the nervous system-wide set of mRNAs localized to presynaptic areas has yet to be identified in any organism. Here we differentially labeled somatic and synaptic compartments in adult C. elegans with fluorescent proteins, and isolated synaptic and somatic regions from the same population of animals. We used this technique to determine the nervous system-wide presynaptic transcriptome by deep sequencing. Analysis of the synaptic transcriptome reveals that synaptic transcripts are predicted to have specialized functions in neurons. Differential expression analysis identified 542 genes enriched in synaptic regions relative to somatic regions, with synaptic functions conserved in higher organisms. We find that mRNAs for pumilio RNA-binding proteins are abundant in synaptic regions, which we confirmed through high-sensitivity in situ hybridization. Presynaptic PUMILIOs regulate associative memory. Our approach enables the identification of new mechanisms that regulate synaptic function and behavior.
Publication Date: 30-Dec-2019
Citation: Arey, Rachel N, Kaletsky, Rachel, Murphy, Coleen T. (2019). Nervous system-wide profiling of presynaptic mRNAs reveals regulators of associative memory.. Scientific reports, 9 (1), 20314 - 20314. doi:10.1038/s41598-019-56908-8
DOI: doi:10.1038/s41598-019-56908-8
ISSN: 2045-2322
EISSN: 2045-2322
Pages: 20314 - 20314
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Scientific Reports
Version: Final published version. This is an open access article.

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