Skip to main content

Enhancer additivity and non-additivity are determined by enhancer strength in the Drosophila embryo

Author(s): Bothma, Jacques P.; Garcia, Hernan G.; Ng, Samuel; Perry, Michael W.; Gregor, Thomas; et al

To refer to this page use:
Abstract: Metazoan genes are embedded in a rich milieu of regulatory information that often includes multiple enhancers possessing overlapping activities. In this study, we employ quantitative live imaging methods to assess the function of pairs of primary and shadow enhancers in the regulation of key patterning genes-knirps, hunchback, and snail-in developing Drosophila embryos. The knirps enhancers exhibit additive, sometimes even super-additive activities, consistent with classical gene fusion studies. In contrast, the hunchback enhancers function sub-additively in anterior regions containing saturating levels of the Bicoid activator, but function additively in regions where there are diminishing levels of the Bicoid gradient. Strikingly sub-additive behavior is also observed for snail, whereby removal of the proximal enhancer causes a significant increase in gene expression. Quantitative modeling of enhancer–promoter interactions suggests that weakly active enhancers function additively while strong enhancers behave sub-additively due to competition with the target promoter. © 2015, Siebert et al.
Publication Date: 12-Aug-2015
Citation: Bothma, JP, Garcia, HG, Ng, S, Perry, MW, Gregor, T, Levine, M. (2015). Enhancer additivity and non-additivity are determined by enhancer strength in the Drosophila embryo. eLife, 4 (August 2015), doi:10.7554/eLife.07956.001
DOI: doi:10.7554/eLife.07956.001
EISSN: 2050-084X
Pages: 1 - 14
Type of Material: Journal Article
Journal/Proceeding Title: eLife
Version: Final published version. This is an open access article.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.