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Outer membrane lipoprotein biogenesis: Lol is not the end

Author(s): Konovalova, Anna; Silhavy, Thomas J

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dc.contributor.authorKonovalova, Anna-
dc.contributor.authorSilhavy, Thomas J-
dc.date.accessioned2022-01-25T14:49:48Z-
dc.date.available2022-01-25T14:49:48Z-
dc.date.issued2015-10-05en_US
dc.identifier.citationKonovalova, Anna, Silhavy, Thomas J. (2015). Outer membrane lipoprotein biogenesis: Lol is not the end. Philosophical Transactions of the Royal Society B: Biological Sciences, 370 (1679), 20150030 - 20150030. doi:10.1098/rstb.2015.0030en_US
dc.identifier.issn0962-8436-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1959c70r-
dc.description.abstractBacterial lipoproteins are lipid-anchored proteins that contain acyl groups covalently attached to the N-terminal cysteine residue of the mature protein. Lipoproteins are synthesized in precursor form with an N-terminal signal sequence (SS) that targets translocation across the cytoplasmic or inner membrane (IM). Lipid modification and SS processing take place at the periplasmic face of the IM. Outer membrane (OM) lipoproteins take the localization of lipoproteins (Lol) export pathway, which ends with the insertion of the N-terminal lipid moiety into the inner leaflet of the OM. For many lipoproteins, the biogenesis pathway ends here. We provide examples of lipoproteins that adopt complex topologies in the OM that include transmembrane and surface-exposed domains. Biogenesis of such lipoproteins requires additional steps beyond the Lol pathway. In at least one case, lipoprotein sequences reach the cell surface by being threaded through the lumen of a beta-barrel protein in an assembly reaction that requires the heteropentomeric Bam complex. The inability to predict surface exposure reinforces the importance of experimental verification of lipoprotein topology and we will discuss some of the methods used to study OM protein topology.en_US
dc.format.extent20150030 - 20150030en_US
dc.language.isoen_USen_US
dc.relation.ispartofPhilosophical Transactions of the Royal Society B: Biological Sciencesen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleOuter membrane lipoprotein biogenesis: Lol is not the enden_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1098/rstb.2015.0030-
dc.date.eissued2015-09-14en_US
dc.identifier.eissn1471-2970-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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