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Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields

Author(s): Rickgauer, John Peter; Deisseroth, Karl; Tank, David W

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Abstract: Linking neural microcircuit function to emergent properties of the mammalian brain requires finescale manipulation and measurement of neural activity during behavior, where each neuron’s coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, λ = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, λ = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or ‘biasing’, to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields.
Publication Date: Dec-2014
Electronic Publication Date: 17-Nov-2014
Citation: Rickgauer, John Peter, Deisseroth, Karl, Tank, David W. (2014). Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields. Nature Neuroscience, 17 (12), 1816 - 1824. doi:10.1038/nn.3866
DOI: doi:10.1038/nn.3866
ISSN: 1097-6256
EISSN: 1546-1726
Pages: 1816 - 1824
Type of Material: Journal Article
Journal/Proceeding Title: Nature Neuroscience
Version: Author's manuscript

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