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The structural basis of Rubisco phase separation in the pyrenoid

Author(s): He, Shan; Chou, Hui-Ting; Matthies, Doreen; Wunder, Tobias; Meyer, Moritz T; et al

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dc.contributor.authorHe, Shan-
dc.contributor.authorChou, Hui-Ting-
dc.contributor.authorMatthies, Doreen-
dc.contributor.authorWunder, Tobias-
dc.contributor.authorMeyer, Moritz T-
dc.contributor.authorAtkinson, Nicky-
dc.contributor.authorMartinez-Sanchez, Antonio-
dc.contributor.authorJeffrey, Philip D-
dc.contributor.authorPort, Sarah A-
dc.contributor.authorPatena, Weronika-
dc.contributor.authorHe, Guanhua-
dc.contributor.authorChen, Vivian K-
dc.contributor.authorHughson, Frederick M-
dc.contributor.authorMcCormick, Alistair J-
dc.contributor.authorMueller-Cajar, Oliver-
dc.contributor.authorEngel, Benjamin D-
dc.contributor.authorYu, Zhiheng-
dc.contributor.authorJonikas, Martin C-
dc.date.accessioned2023-12-11T18:22:19Z-
dc.date.available2023-12-11T18:22:19Z-
dc.date.issued2020-12en_US
dc.identifier.citationHe, Shan, Chou, Hui-Ting, Matthies, Doreen, Wunder, Tobias, Meyer, Moritz T, Atkinson, Nicky, Martinez-Sanchez, Antonio, Jeffrey, Philip D, Port, Sarah A, Patena, Weronika, He, Guanhua, Chen, Vivian K, Hughson, Frederick M, McCormick, Alistair J, Mueller-Cajar, Oliver, Engel, Benjamin D, Yu, Zhiheng, Jonikas, Martin C. (2020). The structural basis of Rubisco phase separation in the pyrenoid. Nature plants, 6 (12), 1480 - 1490. doi:10.1038/s41477-020-00811-yen_US
dc.identifier.issn2055-0278-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr15q4rm0m-
dc.description.abstractApproximately one-third of global CO2 fixation occurs in a phase-separated algal organelle called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase separation of the CO2-fixing enzyme Rubisco with a linker protein; however, the molecular interactions underlying this phase separation remain unknown. Here we present the structural basis of the interactions between Rubisco and its intrinsically disordered linker protein Essential Pyrenoid Component 1 (EPYC1) in the model alga Chlamydomonas reinhardtii. We find that EPYC1 consists of five evenly spaced Rubisco-binding regions that share sequence similarity. Single-particle cryo-electron microscopy of these regions in complex with Rubisco indicates that each Rubisco holoenzyme has eight binding sites for EPYC1, one on each Rubisco small subunit. Interface mutations disrupt binding, phase separation and pyrenoid formation. Cryo-electron tomography supports a model in which EPYC1 and Rubisco form a codependent multivalent network of specific low-affinity bonds, giving the matrix liquid-like properties. Our results advance the structural and functional understanding of the phase separation underlying the pyrenoid, an organelle that plays a fundamental role in the global carbon cycle.en_US
dc.format.extent1480 - 1490en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.relation.ispartofNature Plantsen_US
dc.rightsAuthor's manuscripten_US
dc.titleThe structural basis of Rubisco phase separation in the pyrenoiden_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1038/s41477-020-00811-y-
dc.identifier.eissn2055-0278-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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