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Rapid Rates of Pol II Elongation in the Drosophila Embryo

Author(s): Fukaya, Takashi; Lim, Bomyi; Levine, Michael

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Abstract: Elongation of RNA polymerase II (Pol II) is thought to be an important mechanism for regulating gene expression [1]. We measured the first wave of de novo transcription in living Drosophila embryos using dual-fluorescence detection of nascent transcripts containing 5' MS2 and 3' PP7 RNA stem loops. Pol II elongation rates of 2.4-3.0 kb/min were observed, approximately twice as fast as earlier estimates [2-6]. The revised rates permit substantial levels of zygotic gene activity prior to the mid-blastula transition. We also provide evidence that variable rates of elongation are not a significant source of differential gene activity, suggesting that transcription initiation and Pol II release are the key determinants of gene control in development.
Publication Date: 8-May-2017
Citation: Fukaya, Takashi, Lim, Bomyi, Levine, Michael. (2017). Rapid Rates of Pol II Elongation in the Drosophila Embryo.. Current biology : CB, 27 (9), 1387 - 1391. doi:10.1016/j.cub.2017.03.069
DOI: doi:10.1016/j.cub.2017.03.069
ISSN: 0960-9822
EISSN: 1879-0445
Pages: 1387 - 1391
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Current Biology
Version: Author's manuscript

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