Skip to main content

The effect of antibiotics on protein diffusion in the Escherichia coli cytoplasmic membrane.

Author(s): Liu, George S; Bratton, Benjamin P; Gitai, Zemer; Shaevitz, Joshua W

To refer to this page use:
Abstract: Accumulating evidence suggests that molecular motors contribute to the apparent diffusion of molecules in cells. However, current literature lacks evidence for an active process that drives diffusive-like motion in the bacterial membrane. One possible mechanism is cell wall synthesis, which involves the movement of protein complexes in the cell membrane circumferentially around the cell envelope and may generate currents in the lipid bilayer that advectively transport other transmembrane proteins. We test this hypothesis in Escherichia coli using drug treatments that slow cell wall synthesis and measure their effect on the diffusion of the transmembrane protein mannitol permease using fluorescence recovery after photobleaching. We found no clear decrease in diffusion in response to vancomycin and no decrease in response to mecillinam treatment. These results suggest that cell wall synthesis is not an active contributor to mobility in the cytoplasmic membrane.
Publication Date: 4-Oct-2017
Citation: Liu, George S, Bratton, Benjamin P, Gitai, Zemer, Shaevitz, Joshua W. (2017). The effect of antibiotics on protein diffusion in the Escherichia coli cytoplasmic membrane.. PloS one, 12 (10), e0185810 - ?. doi:10.1371/journal.pone.0185810
DOI: doi:10.1371/journal.pone.0185810
ISSN: 1932-6203
EISSN: 1932-6203
Pages: 1 - 11
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: PloS one
Version: Final published version. This is an open access article.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.