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Mechanism of how augmin directly targets the γ-tubulin ring complex to microtubules

Author(s): Song, Jae-Geun; King, Matthew R; Zhang, Rui; Kadzik, Rachel S; Thawani, Akanksha; et al

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dc.contributor.authorSong, Jae-Geun-
dc.contributor.authorKing, Matthew R-
dc.contributor.authorZhang, Rui-
dc.contributor.authorKadzik, Rachel S-
dc.contributor.authorThawani, Akanksha-
dc.contributor.authorPetry, Sabine-
dc.date.accessioned2022-01-25T14:51:29Z-
dc.date.available2022-01-25T14:51:29Z-
dc.date.issued2018-07en_US
dc.identifier.citationSong, Jae-Geun, King, Matthew R, Zhang, Rui, Kadzik, Rachel S, Thawani, Akanksha, Petry, Sabine. (2018). Mechanism of how augmin directly targets the γ-tubulin ring complex to microtubules. The Journal of Cell Biology, 217 (7), 2417 - 2428. doi:10.1083/jcb.201711090en_US
dc.identifier.issn0021-9525-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr13j3909r-
dc.description.abstractMicrotubules (MTs) must be generated from precise locations to form the structural frameworks required for cell shape and function. MTs are nucleated by the γ-tubulin ring complex (γ-TuRC), but it remains unclear how γ-TuRC gets to the right location. Augmin has been suggested to be a γ-TuRC targeting factor and is required for MT nucleation from preexisting MTs. To determine augmin's architecture and function, we purified Xenopus laevis augmin from insect cells. We demonstrate that augmin is sufficient to target γ-TuRC to MTs by in vitro reconstitution. Augmin is composed of two functional parts. One module (tetramer-II) is necessary for MT binding, whereas the other (tetramer-III) interacts with γ-TuRC. Negative-stain electron microscopy reveals that both tetramers fit into the Y-shape of augmin, and MT branching assays reveal that both are necessary for MT nucleation. The finding that augmin can directly bridge MTs with γ-TuRC via these two tetramers adds to our mechanistic understanding of how MTs can be nucleated from preexisting MTs.en_US
dc.format.extent2417 - 2428en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.relation.ispartofThe Journal of Cell Biologyen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleMechanism of how augmin directly targets the γ-tubulin ring complex to microtubulesen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1083/jcb.201711090-
dc.identifier.eissn1540-8140-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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