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Mapping the pericentric heterochromatin by comparative genomic hybridization analysis and chromosome deletions in Drosophila melanogaster.

Author(s): He, Bing; Caudy, Amy; Parsons, Lance; Rosebrock, Adam; Pane, Attilio; et al

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dc.contributor.authorHe, Bing-
dc.contributor.authorCaudy, Amy-
dc.contributor.authorParsons, Lance-
dc.contributor.authorRosebrock, Adam-
dc.contributor.authorPane, Attilio-
dc.contributor.authorRaj, Sandeep-
dc.contributor.authorWieschaus, Eric-
dc.date.accessioned2022-01-25T14:57:56Z-
dc.date.available2022-01-25T14:57:56Z-
dc.date.issued2012en_US
dc.identifier.citationHe, Bing, Caudy, Amy, Parsons, Lance, Rosebrock, Adam, Pane, Attilio, Raj, Sandeep, Wieschaus, Eric. (2012). Mapping the pericentric heterochromatin by comparative genomic hybridization analysis and chromosome deletions in Drosophila melanogaster.. Genome research, 22 (12), 2507 - 2519. doi:10.1101/gr.137406.112en_US
dc.identifier.issn1088-9051-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1319s25q-
dc.description.abstractHeterochromatin represents a significant portion of eukaryotic genomes and has essential structural and regulatory functions. Its molecular organization is largely unknown due to difficulties in sequencing through and assembling repetitive sequences enriched in the heterochromatin. Here we developed a novel strategy using chromosomal rearrangements and embryonic phenotypes to position unmapped Drosophila melanogaster heterochromatic sequence to specific chromosomal regions. By excluding sequences that can be mapped to the assembled euchromatic arms, we identified sequences that are specific to heterochromatin and used them to design heterochromatin specific probes (“H-probes”) for microarray. By comparative genomic hybridization (CGH) analyses of embryos deficient for each chromosome or chromosome arm, we were able to map most of our H-probes to specific chromosome arms. We also positioned sequences mapped to the second and X chromosomes to finer intervals by analyzing smaller deletions with breakpoints in heterochromatin. Using this approach, we were able to map >40% (13.9 Mb) of the previously unmapped heterochromatin sequences assembled by the whole-genome sequencing effort on arm U and arm Uextra to specific locations. We also identified and mapped 110 kb of novel heterochromatic sequences. Subsequent analyses revealed that sequences located within different heterochromatic regions have distinct properties, such as sequence composition, degree of repetitiveness, and level of underreplication in polytenized tissues. Surprisingly, although heterochromatin is generally considered to be transcriptionally silent, we detected region-specific temporal patterns of transcription in heterochromatin during oogenesis and early embryonic development. Our study provides a useful approach to elucidate the molecular organization and function of heterochromatin and reveals region-specific variation of heterochromatin.en_US
dc.format.extent2507 - 2519en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.relation.ispartofGenome Researchen_US
dc.rightsFinal published version. Article is made available in OAR by the publisher's permission or policy.en_US
dc.titleMapping the pericentric heterochromatin by comparative genomic hybridization analysis and chromosome deletions in Drosophila melanogaster.en_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1101/gr.137406.112-
dc.date.eissued2012-06-28en_US
dc.identifier.eissn1549-5469-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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