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Structural Basis of Vesicle Formation at the Inner Nuclear Membrane

Author(s): Hagen, Christoph; Dent, Kyle C; Zeev-Ben-Mordehai, Tzviya; Grange, Michael; Bosse, Jens B; et al

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dc.contributor.authorHagen, Christoph-
dc.contributor.authorDent, Kyle C-
dc.contributor.authorZeev-Ben-Mordehai, Tzviya-
dc.contributor.authorGrange, Michael-
dc.contributor.authorBosse, Jens B-
dc.contributor.authorWhittle, Cathy-
dc.contributor.authorKlupp, Barbara G-
dc.contributor.authorSiebert, C Alistair-
dc.contributor.authorVasishtan, Daven-
dc.contributor.authorBäuerlein, Felix JB-
dc.contributor.authorCheleski, Juliana-
dc.contributor.authorWerner, Stephan-
dc.contributor.authorGuttmann, Peter-
dc.contributor.authorRehbein, Stefan-
dc.contributor.authorHenzler, Katja-
dc.contributor.authorDemmerle, Justin-
dc.contributor.authorAdler, Barbara-
dc.contributor.authorKoszinowski, Ulrich-
dc.contributor.authorSchermelleh, Lothar-
dc.contributor.authorSchneider, Gerd-
dc.contributor.authorEnquist, Lynn W-
dc.contributor.authorPlitzko, Jürgen M-
dc.contributor.authorMettenleiter, Thomas C-
dc.contributor.authorGrünewald, Kay-
dc.identifier.citationHagen, Christoph, Dent, Kyle C, Zeev-Ben-Mordehai, Tzviya, Grange, Michael, Bosse, Jens B, Whittle, Cathy, Klupp, Barbara G, Siebert, C Alistair, Vasishtan, Daven, Bäuerlein, Felix JB, Cheleski, Juliana, Werner, Stephan, Guttmann, Peter, Rehbein, Stefan, Henzler, Katja, Demmerle, Justin, Adler, Barbara, Koszinowski, Ulrich, Schermelleh, Lothar, Schneider, Gerd, Enquist, Lynn W, Plitzko, Jürgen M, Mettenleiter, Thomas C, Grünewald, Kay. (2015). Structural Basis of Vesicle Formation at the Inner Nuclear Membrane. Cell, 163 (7), 1692 - 1701. doi:10.1016/j.cell.2015.11.029en_US
dc.description.abstractVesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope. Cargo is recruited, enveloped at the inner nuclear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understand the structural underpinning for this trafficking, we investigated nuclear egress of progeny herpesvirus capsids where capsid envelopment is mediated by two viral proteins, forming the nuclear egress complex (NEC). Using a multi-modal imaging approach, we visualized the NEC in situ forming coated vesicles of defined size. Cellular electron cryo-tomography revealed a protein layer showing two distinct hexagonal lattices at its membrane-proximal and membrane-distant faces, respectively. NEC coat architecture was determined by combining this information with integrative modeling using small-angle X-ray scattering data. The molecular arrangement of the NEC establishes the basic mechanism for budding and scission of tailored vesicles at the INM.en_US
dc.format.extent1692 - 1701en_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleStructural Basis of Vesicle Formation at the Inner Nuclear Membraneen_US
dc.typeJournal Articleen_US

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