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BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila

Author(s): Ueberschär, Malin; Wang, Huazhen; Zhang, Chun; Kondo, Shu; Aoki, Tsutomu; et al

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Abstract: The Drosophila genome encodes three BEN-solo proteins including Insensitive (Insv), Elba1 and Elba2 that possess activities in transcriptional repression and chromatin insulation. A fourth protein-Elba3-bridges Elba1 and Elba2 to form an ELBA complex. Here, we report comprehensive investigation of these proteins in Drosophila embryos. We assess common and distinct binding sites for Insv and ELBA and their genetic interdependencies. While Elba1 and Elba2 binding generally requires the ELBA complex, Elba3 can associate with chromatin independently of Elba1 and Elba2. We further demonstrate that ELBA collaborates with other insulators to regulate developmental patterning. Finally, we find that adjacent gene pairs separated by an ELBA bound sequence become less differentially expressed in ELBA mutants. Transgenic reporters confirm the insulating activity of ELBA- and Insv-bound sites. These findings define ELBA and Insv as general insulator proteins in Drosophila and demonstrate the functional importance of insulators to partition transcription units.
Publication Date: 13-Dec-2019
Citation: Ueberschär, Malin, Wang, Huazhen, Zhang, Chun, Kondo, Shu, Aoki, Tsutomu, Schedl, Paul, Lai, Eric C, Wen, Jiayu, Dai, Qi. (2019). BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila. Nature communications, 10 (1), 5700 - 5700. doi:10.1038/s41467-019-13558-8
DOI: doi:10.1038/s41467-019-13558-8
ISSN: 2041-1723
EISSN: 2041-1723
Pages: 5700 - 5700
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: Nature Communications
Version: Final published version. This is an open access article.

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