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The yeast genome undergoes significant topological reorganization in quiescence

Author(s): Rutledge, Mark T; Russo, Mariano; Belton, Jon-Matthew; Dekker, Job; Broach, James R

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Abstract: We have examined the three-dimensional organization of the yeast genome during quiescence by a chromosome capture technique as a means of understanding how genome organization changes during development. For exponentially growing cells we observe high levels of inter-centromeric interaction but otherwise a predominance of intrachromosomal interactions over interchromosomal interactions, consistent with aggregation of centromeres at the spindle pole body and compartmentalization of individual chromosomes within the nucleoplasm. Three major changes occur in the organization of the quiescent cell genome. First, intrachromosomal associations increase at longer distances in quiescence as compared to growing cells. This suggests that chromosomes undergo condensation inquiescence, which we confirmed by microscopy by measurement of the intrachromosomal distances between two sites on one chromosome. This compaction in quiescence requires the condensin complex. Second, inter-centromeric interactions decrease, consistent with prior data indicating that centromeres disperse along an array of microtubules during quiescence. Third, inter-telomeric interactions significantly increase in quiescence, an observation also confirmed by direct measurement. Thus, survival during quiescence is associated with substantial topological reorganization of the genome.
Publication Date: 30-Sep-2015
Electronic Publication Date: 21-Jul-2015
Citation: Rutledge, Mark T, Russo, Mariano, Belton, Jon-Matthew, Dekker, Job, Broach, James R. (2015). The yeast genome undergoes significant topological reorganization in quiescence. Nucleic Acids Research, 43 (17), 8299 - 8313. doi:10.1093/nar/gkv723
DOI: doi:10.1093/nar/gkv723
ISSN: 0305-1048
EISSN: 1362-4962
Pages: 1 - 15
Type of Material: Journal Article
Journal/Proceeding Title: Nucleic Acids Research
Version: Final published version. Article is made available in OAR by the publisher's permission or policy.

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