Skip to main content

The CPEB translational regulator, Orb, functions together with Par proteins to polarize the Drosophila oocyte

Author(s): Barr, Justinn; Charania, Sofia; Gilmutdinov, Rudolf; Yakovlev, Konstantin; Shidlovskii, Yulii; et al

To refer to this page use:
Abstract: orb is a founding member of the CPEB family of translational regulators and is required at multiple steps during Drosophila oogenesis. Previous studies showed that orb is required during mid-oogenesis for the translation of the posterior/germline determinant oskar mRNA and the dorsal-ventral determinant gurken mRNA. Here, we report that orb also functions upstream of these axes determinants in the polarization of the microtubule network (MT). Prior to oskar and gurken translational activation, the oocyte MT network is repolarized. The MT organizing center at the oocyte posterior is disassembled, and a new MT network is established at the oocyte anterior. Repolarization depends upon cross-regulatory interactions between anterior (apical) and posterior (basal) Par proteins. We show that repolarization of the oocyte also requires orb and that orb is needed for the proper functioning of the Par proteins. orb interacts genetically with aPKC and cdc42 and in egg chambers compromised for orb activity, Par-1 and aPKC protein and aPKC mRNA are mislocalized. Moreover, like cdc42-, the defects in Par protein localization appear to be connected to abnormalities in the cortical actin cytoskeleton. These abnormalities also disrupt the localization of the spectraplakin Shot and the microtubule minus-end binding protein Patronin. These two proteins play a critical role in the repolarization of the MT network.
Publication Date: 13-Mar-2019
Citation: Barr, Justinn, Charania, Sofia, Gilmutdinov, Rudolf, Yakovlev, Konstantin, Shidlovskii, Yulii, Schedl, Paul. (2019). The CPEB translational regulator, Orb, functions together with Par proteins to polarize the Drosophila oocyte. PLoS Genetics, 15 (3), e1008012 - e1008012. doi:10.1371/journal.pgen.1008012
DOI: doi:10.1371/journal.pgen.1008012
ISSN: 1553-7390
EISSN: 1553-7404
Pages: e1008012 - e1008012
Language: eng
Type of Material: Journal Article
Journal/Proceeding Title: PLoS Genetics
Version: Final published version. This is an open access article.

Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.