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|Abstract:||Understanding the genetic basis of gene regulatory variation is a key goal of evolutionary and medical genetics. Regulatory variation can act in an allele-specific manner (cis-acting) or it can affect both alleles of a gene (trans-acting). Differential allele-specific expression (ASE), in which the expression of one allele differs from another in a diploid, implies the presence of cis-acting regulatory variation. While microarrays and high-throughput sequencing have enabled genome-wide measurements of transcriptional ASE, methods for measurement of protein ASE (pASE) have lagged far behind. We describe a flexible, accurate, and scalable strategy for measurement of pASE by liquid chromatography-coupled mass spectrometry (LC-MS). We apply this approach to a hybrid between the yeast species Saccharomyces cerevisiae and Saccharomyces bayanus. Our results provide the first analysis of the relative contribution of cis-acting and trans-acting regulatory differences to protein expression divergence between yeast species.|
|Electronic Publication Date:||14-Aug-2012|
|Citation:||Khan, Z, Bloom, JS, Amini, S, Singh, M, Perlman, DH, Caudy, AA, Kruglyak, L. (2012). Quantitative measurement of allele-specific protein expression in a diploid yeast hybrid by LC-MS. Molecular Systems Biology, 8 (10.1038/msb.2012.34|
|Type of Material:||Journal Article|
|Journal/Proceeding Title:||Molecular Systems Biology|
|Version:||Final published version. Article is made available in OAR by the publisher's permission or policy.|
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