To refer to this page use:
|Abstract:||By exploiting photonic reagents (i.e., coherent control by shaped laser pulses), we employ Optimal Dynamic Discrimination (ODD) as a novel means for quantitatively characterizing mixtures of fluorescent proteins with a large spectral overlap. To illustrate ODD, we simultaneously measured concentrations of in vitro mixtures of Enhanced Blue Fluorescent Protein (EBFP) and Enhanced Cyan Fluorescent Protein (ECFP). Building on this foundational study, the ultimate goal is to exploit the capabilities of ODD for parallel monitoring of genetic and protein circuits by suppressing the spectral cross-talk among multiple fluorescent reporters.|
|Citation:||Goun, Alexei, Bondar, Denys I., Er, Ali O., Quine, Zachary, Rabitz, Herschel A. (2016). Photonic reagents for concentration measurement of fluorescent proteins with overlapping spectra. SCIENTIFIC REPORTS, 6 (10.1038/srep25827|
|Pages:||6:25827-1 - 25827-9|
|Type of Material:||Journal Article|
|Journal/Proceeding Title:||SCIENTIFIC REPORTS|
|Version:||Final published version. This is an open access article.|
Items in OAR@Princeton are protected by copyright, with all rights reserved, unless otherwise indicated.