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The insulator functions of the Drosophila polydactyl C2H2 zinc finger protein CTCF: Necessity versus sufficiency

Author(s): Kyrchanova, Olga; Maksimenko, Oksana; Ibragimov, Airat; Sokolov, Vladimir; Postika, Nikolay; et al

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dc.contributor.authorKyrchanova, Olga-
dc.contributor.authorMaksimenko, Oksana-
dc.contributor.authorIbragimov, Airat-
dc.contributor.authorSokolov, Vladimir-
dc.contributor.authorPostika, Nikolay-
dc.contributor.authorLukyanova, Maria-
dc.contributor.authorSchedl, Paul-
dc.contributor.authorGeorgiev, Pavel-
dc.date.accessioned2022-01-25T14:52:43Z-
dc.date.available2022-01-25T14:52:43Z-
dc.date.issued2020-03-25en_US
dc.identifier.citationKyrchanova, Olga, Maksimenko, Oksana, Ibragimov, Airat, Sokolov, Vladimir, Postika, Nikolay, Lukyanova, Maria, Schedl, Paul, Georgiev, Pavel. (2020). The insulator functions of the Drosophila polydactyl C2H2 zinc finger protein CTCF: Necessity versus sufficiency. Science advances, 6 (13), eaaz3152 - eaaz3152. doi:10.1126/sciadv.aaz3152en_US
dc.identifier.issn2375-2548-
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr1rj48t69-
dc.description.abstractIn mammals, a C2H2 zinc finger (C2H2) protein, CTCF, acts as the master regulator of chromosomal architecture and of the expression of Hox gene clusters. Like mammalian CTCF, the Drosophila homolog, dCTCF, localizes to boundaries in the bithorax complex (BX-C). Here, we have determined the minimal requirements for the assembly of a functional boundary by dCTCF and two other C2H2 zinc finger proteins, Pita and Su(Hw). Although binding sites for these proteins are essential for the insulator activity of BX-C boundaries, these binding sites alone are insufficient to create a functional boundary. dCTCF cannot effectively bind to a single recognition sequence in chromatin or generate a functional insulator without the help of additional proteins. In addition, for boundary elements in BX-C at least four binding sites for dCTCF or the presence of additional DNA binding factors is required to generate a functional insulator.en_US
dc.format.extenteaaz3152 - eaaz3152en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.relation.ispartofScience Advancesen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleThe insulator functions of the Drosophila polydactyl C2H2 zinc finger protein CTCF: Necessity versus sufficiencyen_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1126/sciadv.aaz3152-
dc.identifier.eissn2375-2548-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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