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Functional Genome Annotation of Drosophila Seminal Fluid Proteins Using Transcriptional Genetic Networks

Author(s): Ayroles, Julien F.; Laflamme, Brooke A.; Stone, Eric A.; Wolfner, Mariana F.; Mackay, Trudy F. C.

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Abstract: Predicting functional gene annotations remains a significant challenge, even in well-annotated genomes such as yeast and Drosophila. One promising, high-throughput method for gene annotation is to use correlated gene expression patterns to annotate target genes based on the known function of focal genes. The Drosophila melanogaster transcriptome varies genetically among wild derived inbred lines, with strong genetic correlations among the transcripts. Here, we leveraged the genetic correlations in gene expression among known seminal fluid protein (SFP) genes and the rest of the genetically varying transcriptome to identify 176 novel candidate SFPs (cSFPs). We independently validated the correlation in gene expression between seven of the cSFPs and a known SFP gene, as well as expression in male reproductive tissues. We argue that this method can be extended to other systems for which information on genetic variation in gene expression is available.
Publication Date: Dec-2011
Electronic Publication Date: 22-Dec-2011
Citation: Ayroles, Julien F., Laflamme, Brooke A., Stone, Eric A., Wolfner, Mariana F., Mackay, Trudy F. C. (2011). Functional Genome Annotation of Drosophila Seminal Fluid Proteins Using Transcriptional Genetic Networks. Genetics Research, 93 (06), 387 - 395. doi:10.1017/S0016672311000346
DOI: doi:10.1017/S0016672311000346
ISSN: 0016-6723
EISSN: 1469-5073
Pages: 387 - 395
Type of Material: Journal Article
Journal/Proceeding Title: Genetics Research
Version: Author's manuscript



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