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|Abstract:||Because off-target effects hamper interpretation and validation of RNAi screen data, we developed a bioinformatics method, genome-wide enrichment of seed sequence matches (GESS), to identify candidate off-targeted transcripts in primary screening data. GESS analysis revealed a prominent off-targeted transcript in several screens, including MAD2 (MAD2L1) in a screen for genes required for the spindle assembly checkpoint. GESS analysis results can enhance the validation rate in RNAi screens.|
|Citation:||Sigoillot, Frederic D, Lyman, Susan, Huckins, Jeremy F, Adamson, Britt, Chung, Eunah, Quattrochi, Brian, King, Randall W. (2012). A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens.. Nature methods, 9 (4), 363 - 366. doi:10.1038/nmeth.1898|
|Pages:||1 - 7|
|Type of Material:||Journal Article|
|Journal/Proceeding Title:||Nature methods|
|Version:||Final published version. Article is made available in OAR by the publisher's permission or policy.|
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