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A Copy Number Variant at the KITLG Locus Likely Confers Risk for Canine Squamous Cell Carcinoma of the Digit

Author(s): Karyadi, Danielle M.; Karlins, Eric; Decker, Brennan; vonHoldt, Bridgett M.; Carpintero-Ramirez, Gretchen; et al

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dc.contributor.authorKaryadi, Danielle M.-
dc.contributor.authorKarlins, Eric-
dc.contributor.authorDecker, Brennan-
dc.contributor.authorvonHoldt, Bridgett M.-
dc.contributor.authorCarpintero-Ramirez, Gretchen-
dc.contributor.authorParker, Heidi G.-
dc.contributor.authorWayne, Robert K.-
dc.contributor.authorOstrander, Elaine A.-
dc.date.accessioned2019-05-06T18:08:51Z-
dc.date.available2019-05-06T18:08:51Z-
dc.date.issued2013-03-28en_US
dc.identifier.citationKaryadi, Danielle M, Karlins, Eric, Decker, Brennan, vonHoldt, Bridgett M, Carpintero-Ramirez, Gretchen, Parker, Heidi G, Wayne, Robert K, Ostrander, Elaine A. (2013). A Copy Number Variant at the KITLG Locus Likely Confers Risk for Canine Squamous Cell Carcinoma of the Digit. PLoS Genetics, 9 (3), e1003409 - e1003409. doi:10.1371/journal.pgen.1003409en_US
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/pr11d9b-
dc.description.abstractThe domestic dog is a robust model for studying the genetics of complex disease susceptibility. The strategies used to develop and propagate modern breeds have resulted in an elevated risk for specific diseases in particular breeds. One example is that of Standard Poodles (STPOs), who have increased risk for squamous cell carcinoma of the digit (SCCD), a locally aggressive cancer that causes lytic bone lesions, sometimes with multiple toe recurrence. However, only STPOs of dark coat color are at high risk; light colored STPOs are almost entirely unaffected, suggesting that interactions between multiple pathways are necessary for oncogenesis. We performed a genome-wide association study (GWAS) on STPOs, comparing 31 SCCD cases to 34 unrelated black STPO controls. The peak SNP on canine chromosome 15 was statistically significant at the genome-wide level (Praw = 1.6061027 ; Pgenome = 0.0066). Additional mapping resolved the region to the KIT Ligand (KITLG) locus. Comparison of STPO cases to other at-risk breeds narrowed the locus to a 144.9-Kb region. Haplotype mapping among 84 STPO cases identified a minimal region of 28.3 Kb. A copy number variant (CNV) containing predicted enhancer elements was found to be strongly associated with SCCD in STPOs (P = 1.7261028). Light colored STPOs carry the CNV risk alleles at the same frequency as black STPOs, but are not susceptible to SCCD. A GWAS comparing 24 black and 24 light colored STPOs highlighted only the MC1R locus as significantly different between the two datasets, suggesting that a compensatory mutation within the MC1R locus likely protects light colored STPOs from disease. Our findings highlight a role for KITLG in SCCD susceptibility, as well as demonstrate that interactions between the KITLG and MC1R loci are potentially required for SCCD oncogenesis. These findings highlight how studies of breed-limited diseases are useful for disentangling multigene disorders.en_US
dc.format.extente1003409 - e1003409en_US
dc.language.isoen_USen_US
dc.relation.ispartofPLoS Geneticsen_US
dc.rightsFinal published version. This is an open access article.en_US
dc.titleA Copy Number Variant at the KITLG Locus Likely Confers Risk for Canine Squamous Cell Carcinoma of the Digiten_US
dc.typeJournal Articleen_US
dc.identifier.doidoi:10.1371/journal.pgen.1003409-
dc.date.eissued2013-03-28en_US
dc.identifier.eissn1553-7404-
pu.type.symplectichttp://www.symplectic.co.uk/publications/atom-terms/1.0/journal-articleen_US

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